Involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM). Catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) (PubMed:15939292). PimA plays an essential role for growth in macrophages and during both the acute and chronic phases of infection (PubMed:25049093).
10 20 30 40 50
MRIGMICPYS FDVPGGVQSH VLQLAEVMRT RGHLVSVLAP ASPHAALPDY
60 70 80 90 100
FVSGGRAVPI PYNGSVARLR FGPATHRKVK KWLAHGDFDV LHLHEPNAPS
110 120 130 140 150
LSMLALNIAE GPIVATFHTS TTKSLTLTVF QGILRPMHEK IVGRIAVSDL
160 170 180 190 200
ARRWQMEALG SDAVEIPNGV DVDSFASAAR LDGYPRQGKT VLFLGRYDEP
210 220 230 240 250
RKGMAVLLDA LPKVVQRFPD VQLLIVGHGD ADQLRGQAGR LAAHLRFLGQ
260 270 280 290 300
VDDAGKASAM RSADVYCAPN TGGESFGIVL VEAMAAGTAV VASDLDAFRR
310 320 330 340 350
VLRDGEVGHL VPVDPPDLQA AALADGLIAV LENDVLRERY VAAGNAAVRR
360 370 380
YDWSVVASQI MRVYETVAGS GAKVQVAS
1. Expression and purification of a functionally active recombinant GDP-mannosyltransferase (PimA) from Mycobacterium tuberculosis H37Rv.
PubMed:
15939292
doi:10.1016/j.pep.2005.03.015
2. targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.
PubMed:
19099550
doi:10.1186/1752-0509-2-109
3. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
PubMed:
21969609
doi:10.1074/mcp.M111.011445
4. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
PubMed:
21969609
doi:10.1074/mcp.m111.011627
5. The phosphatidyl-myo-inositol mannosyltransferase PimA is essential for Mycobacterium tuberculosis growth in vitro and in vivo.
PubMed:
25049093
doi:10.1128/jb.01346-13
6. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
PubMed:
9634230
doi:10.1038/31159